RPTPγ (PTPRG), GST-tag

Catalog #: 30047
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 65.6 kDa
Genbank #: NM_002841
Tag(s): N-terminal GST-tag
a.a: 801–1147
Background:
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.
Description:
Protein tyrosine phosphatase PTPRG, also known as PTPγ (gamma) (GenBank accession No. NM_002841), a.a. 801-1147 (catalytic domain) with N-terminal GST-tag, MW=65.6 kDa, expressed in an E. coli expression system.
Synonym(s):
RPTgamma, PTPRG
Specific Activity:
560 pmole/min/μg
Unit Definition:
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions:
20 mM HEPES, pH 7.2, 2 mM EDTA, 1 mM DTT, and 50 mM pNPP. Reaction was done at RT for 5 min, and monitored at Abs405 nm continuously. (Path length is estimated to 0.5 cm)  of pNPP = 18,000 M-1cm-1
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. C. Van Niekerk and L. Poels Cancer Lett. 1999, 137: 61.
2. S. Liu, et al., Anticancer Res. 2002, 22:3917.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Receptor
Product Type:
Recombinant Protein
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Product Datasheets

30047_140911.pdf