RPTPγ (PTPRG), GST-tag Recombinant

Catalog #

30047

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase PTPRG, also known as PTPγ (gamma) (GenBank accession No. NM_002841), a.a. 801-1147 (catalytic domain) with N-terminal GST-tag, MW=65.6 kDa, expressed in an E. coli expression system.

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Synonyms

RPTgamma, PTPRG

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione

65.6 kDa

Amino Acids

801–1147

Specific Activity

560 pmole/min/μg

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Genbank #

NM_002841

UniProt #

P23470

Tag(s)

N-terminal GST-tag

Background

PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.

References

1. C. Van Niekerk and L. Poels Cancer Lett. 1999, 137: 61.
2. S. Liu, et al., Anticancer Res. 2002, 22:3917.

Storage/Stability

>12 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 2 mM EDTA, 1 mM DTT, and 50 mM pNPP. Reaction was done at RT for 5 min, and monitored at Abs405 nm continuously. (Path length is estimated to 0.5 cm) of pNPP = 18,000 M-1cm-1

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Receptor

Regulatory Status

Research Use Only