Ret, GST-tag Recombinant

Catalog #

40267

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Ret (GenBank Accession No. NM_020975), a.a. 658-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

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Synonyms

RET, CDHF, CDHR, Cadherin-Related Family Member 16, Hydroxyaryl-Protein Kinase, MEN2A, Cadherin Family Member 12

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.

77.5 kDa

Amino Acids

658–end

Specific Activity

52 pmol/min/μg.

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Genbank #

NM_020975

UniProt #

P07949

Tag(s)

N-terminal GST-tag

References

1. Moore,S.W., Appfelstaedt,J. and Zaahl,M.G. J. Pediatr. Surg. 42 (2), 326-332 (2007).
2 Griseri,P., Lantieri,F., Puppo,F., Bachetti,T., Di Duca,M., Ravazzolo,R. and Ceccherini,I. Hum. Mutat. 28 (2), 168-176 (2007).

Storage/Stability

>6 months at –80°C.

Assay Conditions

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Ret.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40267_Lot1001.pdf

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Ret, GST-tag Recombinant

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