SIK2, GST-tag Recombinant

Catalog #

40264

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Synonyms

SNF1LK2, QIK, KIAA0781, LOH11CR1I, DKFZp434K1115

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

SIK2 (GST-1-926(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

150 kDa

Amino Acids

1-926(end)

Glycosylation

This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Specific Activity

112 pmol/min/µg

Genbank #

NM_015191

UniProt #

DQ9H0K1

Tag(s)

N-terminal GST-tag

Background

SIK2 is a serine/threonine protein kinase that contains an N-terminal kinase domain, a central domain with ubiquitin-associate motif, and a C-terminal PKA phosphorylation site. SIK2 can phosphorylate IRS1 and overexpression of QIK in adipocyte elevates the phosphorylation of IRS1 (1). The SIK2-mediated phosphorylation of IRS1 may modulate the efficiency of insulin signal transduction and could be responsible for insulin resistance associated with diabetes (1). Insulin disrupts TORC2 activity by induction of SIK2 which then stimulates the phosphorylation and cytoplasmic translocation of TORC2. Phosphorylated TORC2 is subsequently degraded by the 26S proteasome (2).

References

1. Horike N, et al: Adipose-specific expression, phosphorylation of ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 278: 18440-18447, 2003.
2. Dentin R, et al: Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. Nature 449: 366-369, 2007.

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Datasheets/Protocols

40264 Lot 180521

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SIK2, GST-tag Recombinant

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