PYK2, His-tag Recombinant

Catalog #

40480

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

CADTK, CAK beta, CAK-beta, CAKB, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, PKB, Proline-rich tyrosine kinase 2, protein kinase B, protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK, Re

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥62%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

120 kDa

Amino Acids

full length

Specific Activity

5.6 pmol/min/μg.

Genbank #

U33284

UniProt #

Q14289

Tag(s)

N-terminal His-tag

References

1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76.
2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.

Storage/Stability

>6 months at –80°C.

Assay Conditions

ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40480_Lot121203D.pdf

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PYK2, His-tag Recombinant

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