SHP-2 (catalytic domain) Homogeneous Assay Kit
Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. SHP-2 (PTPN11) is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
The SHP-2 (catalytic domain) Homogeneous Assay Kit is a complete assay system designed to measure SHP-2 activity for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is needed to analyze the SHP-2 activity level. The fluorogenic substrate, DiFMUP, is incubated with purified SHP-2 and the enzymatic activity releases DiFMU fluorophore that can then be measured using a fluorescence reader.
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Catalog |
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1 µg |
-80°C |
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50 µl |
-80°C |
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3 ml |
-20°C |
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1mM Na3VO4 |
20 μl |
-80°C |
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79685 |
Black, low binding black microtiter plate |
1 |
Room Temperature |
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Chai, J., et al., Cell, 2001 Mar 9;104(5):769-80.
Denault, JB., and Salvesen, GS., J. Biol. Chem., 2003 Sep 5;278(36):34042-50.