PRMT4 (CARM1), FLAG-tag (HEK293-derived) Recombinant

Catalog #

51047

$450 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.

This product has been cited 4 times.

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Synonyms

PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1, PRMT-4

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PRMT4 inhibition curve using an AlphaLISA assay (#52068). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations4

Species

Human

Host Species/Expression System

FreeStyle 293-F cells

Purity

≥65%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ng/μl FLAG peptide

66 kDa

Amino Acids

2–end

Genbank #

NM_199141

UniProt #

Q86X55

Tag(s)

N-terminal FLAG-tag

Background

CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.

References

1. Li H. et al., J. Biol. Chem. 277 (47), 44623-44630 (2002).
2. Frietze S. et al., Cancer Res. 68 (1), 301-306 (2008).

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (1x HMT Assay Buffer 5, BPS catalog # 52191 and 5-1000 ng methyltransferase PRMT4) is added to wells coated with the substrate on a Thermo HBC Neutravidin black plate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only