PRMT4 (CARM1), FLAG-tag (HEK293-derived)

Catalog #: 51047
Size: 20 µg
$435
Species: Human
Host Species: FreeStyle 293-F cells
MW: 66 kDa
Genbank #: NM_199141
Tag(s): N-terminal FLAG-tag
a.a: 2–end
Background:
CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.
Description:
Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.
UniProt
Q86X55
Synonym(s):
PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1
Assay Conditions:
50 µl reaction mix (1x HMT Assay Buffer 5, BPS catalog # 52191 and 5-1000 ng methyltransferase PRMT4) is added to wells coated with the substrate on a Thermo HBC Neutravidin black plate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ng/μl FLAG peptide
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Li H. et al., J. Biol. Chem. 277 (47), 44623-44630 (2002).
2. Frietze S. et al., Cancer Res. 68 (1), 301-306 (2008).

Application Reference(s):
1. Transition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1 (2017)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51047_Lot130204.pdf