The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4
Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated
PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required
for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample
containing assay buffer and methyltransferase enzyme. Next, primary antibody is added.
Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by
addition of an HRP substrate to produce chemiluminescence that can then be measured
using a chemiluminescence reader.
Kit comes in a convenient format, with 8-well strips precoated with the specific PRMT4 peptide substrate, reagents, and purified PRMT4 enzyme for 96 enzyme reactions.
PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.
References
Dillon S.C., et al. 2005. Genome Biology 6:227.
Storage/Stability
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.