PRMT4 (CARM1), FLAG-tag (HEK293-derived) Recombinant

Catalog #
51047
$450 *
Size: 20 µg
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Description

Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.

This product has been cited 6 times.

Synonyms
PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1, PRMT-4
Product Info
Storage and Usage
Citations6
Species
Human
Host Species/Expression System
FreeStyle 293-F cells
Purity

≥65%

Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ng/μl FLAG peptide
MW
66 kDa
Amino Acids
2–end
Genbank #
NM_199141
UniProt #
Q86X55
Tag(s)
N-terminal FLAG-tag
Background
CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.
References

1. Li H. et al., J. Biol. Chem. 277 (47), 44623-44630 (2002).
2. Frietze S. et al., Cancer Res. 68 (1), 301-306 (2008).