PRMT3 Homogeneous Assay Kit

Catalog #: 52055
Size: 384 reactions
$1,040
Background:
PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Description:
The PRMT3 Homogeneous Assay Kit is designed to measure the activity of PRMT3 for screening and profiling applications, using AlphaLISA® technology. The key to the PRMT3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT3 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
PRMT3, HRMT1L3, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Supplied As:
The PRMT3 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT3 for 384 enzyme reactions.
Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

52055.pdf