PRMT3 Chemiluminescent Assay Kit
The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
This product has been cited 2 times.
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog Number |
Component |
Amount |
Storage |
|
51043 | PRMT3 human recombinant enzyme | 10 µg | -80°C | Avoid freeze/ thaw cycles! |
52120 | 52120 20 μM S-adenosylmethionine* | 250 µl | -80°C | |
52150 | Primary antibody 4 | 100 µl | -80°C | |
52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
52170 | 4x HMT assay buffer 2 | 3 ml | -20°C | |
52100 | Blocking buffer 4 | 50 ml | +4°C | |
HRP chemiluminescent A (transparent bottle) |
6 ml |
+4°C |
||
HRP chemiluminescent B (brown bottle) |
6 ml |
+4°C |
||
96-well plate precoated with histone substrate |
1 plate |
+4°C |
*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to inhibitors.