PRMT3 Chemiluminescent Assay Kit

Catalog #
52005L
$750 *
Size: 96 reactions
Qty
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Description

The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

This product has been cited 2 times.

Synonyms
PRMT3, HRMT1L3, PRMT-3
Product Info
Storage and Usage
Citations2
Assay Kit Format
Chemiluminescent
Species
Human
Supplied As
Kit comes in a convenient format, with a 96-well plate precoated with specific substrate, reagents, and purified PRMT3 enzyme for 100 enzyme reactions.
Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format
Catalog
Number 

Component

Amount 

Storage
51043 PRMT3 human recombinant enzyme 10 µg -80°C





Avoid freeze/

thaw cycles!
52120 52120 20 μM S-adenosylmethionine* 250 µl -80°C
52150 Primary antibody 4 100 µl -80°C
52131H Secondary HRP-labeled antibody 2 10 µl -80°C
52170 4x HMT assay buffer 2 3 ml -20°C
52100 Blocking buffer 4 50 ml +4°C
  HRP chemiluminescent A
(transparent bottle)

6 ml

+4°C
  HRP chemiluminescent B
(brown bottle)

6 ml

+4°C
  96-well plate precoated with histone
substrate

1 plate

+4°C




























*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to inhibitors.

UniProt #
Q8WUV3
References
Dillon S.C., et al. 2005. Genome Biology 6:227.