PARP2 Homogeneous Assay Kit (Discontinued)

Catalog #

80702

$1,470 *

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Purchase

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Description

The PARP2 Homogeneous Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with activated DNA and biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

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Synonyms

poly (ADP-ribose) polymerase 2, poly(ADP-ribosyl)transferase 2, ADPRT, ADPRTL2

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

The PARP2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone substrate, primary antibody, PARP assay buffer, and purified PARP2 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA anti-mIgG acceptor beads, 5 mg/ml (Perkin Elmer #AL105C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (Perkin Elmer #6760002S)
Optiplate -384 (Perkin Elmer #6007290)
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips
0.5 M DTT

Format

Catalog Number	Components	Amount	Storage
80502	PARP2	10 μg	-80°C	*Avoid* *freeze/* *thaw* *cycles!*
	NAD+(750 μM)	400 μl	-20°C
52140K	Primary antibody 11	10 μl	-80°C
	Biotinylated histone substrate*	500 rxns	-80°C
80602A	10x PARP assay buffer**	1 ml	-20°C
52301	4x Detection buffer 1	2 ml	-20°C

*Reconstitute in 500 µl H20
**Add DTT before use

UniProt #

Q9UGN5

Background

PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.

References

Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Scientific Category

PARP

Regulatory Status

Research Use Only

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PARP2 Homogeneous Assay Kit (Discontinued)

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