PFKFB3, His-tag Recombinant

Catalog #

71089

$370 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human 6-phosphofructo-2-kinase/ fructose-2,6-biphosphatase 3, also known as PFKFB3 and IPFK2, (GenBank Accession No. NM_004566), a.a. 2-520(end)) with N-terminal His-tag, MW = 60.4 kDa, expressed in an E. coli cell expression system.

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Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, PFKFB3, PFK2, IPFK2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 360 mM imidazole and 20% glycerol

60.4 kDa

Amino Acids

2–520(end)

Specific Activity

≥28.5 pmole/min/µg.

Genbank #

NM_004566

UniProt #

Q16875

Tag(s)

N-terminal His-tag

References

1. Cavalier, M.C., et al., Proteins 2012;80(4):1143-1153.
2. Reddy, M.M., et al., Leukemia 2012;26(3):481-489.

Storage/Stability

>6 months at –80°C

Assay Conditions

The reaction was done in 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM β-D-fructose-6-phosphate, 100 μM ATP, 2 mM DTT, 5 mM KH2PO4, and various amount of PFKFB3 in a final volume of 50 μL. The reaction was incubated at 30°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo^® Plus reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes, Serine-Threonine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71089_Lot140214-1.pdf

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