PDE9A2, GST-tag Recombinant

Catalog #

60090

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST-tag, MW=87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 34 times.

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Synonyms

PDE9A, phosphodiesterase 9A, PDE9-A2, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE9A2 inhibition curve using a fluorescence polarization assay (#60381). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations34

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20 and 20% Glycerol.

87.7 kDa

Amino Acids

full length

Specific Activity

≥200 pmol/min/µg.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.

Genbank #

NM_001001567

UniProt #

O76083

Tag(s)

N-terminal GST-tag

References

1. Rentero C. and Puigdomenech P., BMC Mol. Biol. 7, 39 (2006).
2. Huai, Q., et al., Journal Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629 (2004).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE9A2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE, Neuroscience

Regulatory Status

Research Use Only