PDE9A2, GST-tag

Catalog #: 60090
Size: 10 µg
Species: Human
Host Species: Sf9 insect cells
MW: 87.7 kDa
Genbank #: NM_001001567
Tag(s): N-terminal GST-tag
a.a: full length
Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST-tag, MW=87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
PDE9A, phosphodiesterase 9A
Specific Activity:
≥200 pmol/min/µg.
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE9A kit, Cat. # 60390. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE9A2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20 and 20% Glycerol.
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rentero C. and Puigdomenech P., BMC Mol. Biol. 7, 39 (2006).
2. Huai, Q., et al., Journal Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629 (2004).

Application Reference(s):
1. Discovery of a Novel Series of Pyrazolo[1,5-a]pyrimidine-Based Phosphodiesterase 2A Inhibitors Structurally Different from N-((1S)-1-(3-Fluoro-4-(trifluoromethoxy)phenyl)-2-methoxyethyl)-7-methoxy-2-oxo-2,3-dihydropyrido[2,3-b]pyrazine-4(1H)-carboxamide (TAK-915), for the Treatment of Cognitive Disorders (2017)
2. Design and Synthesis of Novel and Selective Phosphodiesterase 2 (PDE2a) Inhibitors for the Treatment of Memory Disorders (2017)
3. Discovery of Selective Phosphodiesterase 1 Inhibitors with Memory Enhancing Properties (2017)
4. Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays (2017)
5. Novel N-hydroxybenzamides incorporating 2-oxoindoline with unexpected potent histone deacetylase inhibitory effects and antitumor cytotoxicity. (2017)
6. Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015)
7. Active Site Coupling in PDE:PKA Complexes Promotes Resetting of Mammalian cAMP Signaling (2014)
8. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
9. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
10. Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013)
11. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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