PDE4D (Rat), GST-tag Recombinant

Catalog #

60054

$470 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Rat phosphodiesterase 4D (PDE4D) (GenBank Accession No. NM_001113328), a.a. 2-804 (end) with N-terminal GST-tag, MW=117.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE, phosphodiesterase, PDE4-D, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4D (Rat) inhibition curve using a fluorescence polarization assay (#79629). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol.

117.2 kDa

Amino Acids

2-804 (end)

Specific Activity

≥ 22,535 pmole/min/µg

Genbank #

NM_001113328

UniProt #

P14270

Tag(s)

N-terminal GST-tag

References

1. Leong, H., et al., Life Sci. 2010;86(19-20):740-746.

2. Dodge-Kafka, K.L., et al., J. Biol. Chem. 2010;285(15):11078-11086.

Storage/Stability

>6 months at -80°C

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4D (Rat) at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only