PARP14, GST-tag

Catalog #: 80514
Size: 10 µg
$475
Species: Human
Host Species: Sf9 insect cells
MW: 66.5 kDa
Genbank #: NM_017554
Tag(s): N-terminal GST-tag
a.a: 1470–1801(end)
Description:
Human PARP14 (GenBank Accession No. NM_017554), a.a. 1470-1801(end) with N-terminal His-GST-tag, MW = 66.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q460N5
Synonym(s):
poly (ADP-ribose) polymerase 14, poly(ADP-ribosyl)transferase 14, PARP14
Unit Definition:
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions:
Enzyme reaction is conducted using BPS's PARP14 Assay Kit (Catalog #80568) at room temperature for 1 hour.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 188 mM imidazole, 0.04% Tween-20, 3 mM DTT, 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Barbarulo, A., et al., Oncogene. 10 (1038), 448 (2012).
2. Cho, SH., et al., Proc Natl Acad Sci U S A. 108(38),15972-7 (2011).

Product Specific Reference(s):
1. Wang, P., et al., Tetrahedron. 70(35), 5666–5673 (2014).

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80514_Lot130116_R.pdf