PARP14, His-GST-Tag Recombinant

Catalog #

80514

$515 *

Size: 10 µg

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Description

Recombinant human PARP14 (poly(ADP-ribose) polymerase family member 14), encompassing amino acids 1470-1801 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by a GST-Tag and an optimal TEV protease target sequence that allows cleavage of the tags (TEV: tobacco etch virus cysteine protease #50308). The protein was affinity purified.

This product has been cited 6 times.

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Synonyms

poly (ADP-ribose) polymerase 14, poly(ADP-ribosyl)transferase 14, PARP14, PARP-14, B aggressive lymphoma protein 2, BAL2, ADP-ribosyltransferase diphtheria toxin-like 8, ARTD8, pART8, KIAA1268, PARP-14

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PARP14 Activity

PARP14 inhibition curves using a chemiluminescent assay (#80568). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations6

Species

Human

Construct

PARP14 (His-GST-TEV-1470-1801(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, variable mM imidazole

67 kDa

Amino Acids

1470–1801(end)

Genbank #

NM_017554

UniProt #

Q460N5

Tag(s)

N-terminal His-GST-tag

References

1. Barbarulo, A., et al., Oncogene. 10 (1038), 448 (2012).
2. Cho, SH., et al., Proc Natl Acad Sci U S A. 108(38),15972-7 (2011).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the PARP14 Chemiluminescent Assay Kit (BPS Bioscience #80568) with PARP14 serial titrated 2-fold from 1200 ng – 0 ng.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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Datasheets/Protocols

80514 Lot 210521

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PARP14, His-GST-Tag Recombinant

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