PARP7, FLAG-Tag Recombinant

Catalog #

80527

$540 *

Size: 10 µg

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Description

Recombinant human PARP7 (Poly [ADP-ribose] polymerase 7, also known as TIPARP), encompassing amino acids 400-657(end). This construct contains an N-terminal FLAG-tag. This recombinant protein was affinity purified.

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Synonyms

poly(ADP-ribose) polymerase family member 7, PARP-7, ADP-ribosyltransferase diphtheria toxin-like 14, ARTD14, pART14, TCDD-inducible poly [ADP-ribose] polymerase, TIPARP, PARP-7

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PARP7 Activity

PARP7 inhibition curve using a chemiluminescent assay (#79729-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

PARP7 (FLAG-400-657(end))

Host Species/Expression System

Sf9

Purity

≥55%

Format

Aqueous buffer solution

Formulation

50 mM Tris, pH 8.0, 150 mM NaCl, 0.8 M Arginine, and 10% glycerol

31 kDa

Amino Acids

400-657(end)

Genbank #

NM_015508

UniProt #

Q7Z3E1

Tag(s)

N-terminal FLAG-tag

References

1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.

2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to PARP7 Chemiluminescent Assay Kit (BPS Bioscience #79729) with PARP7 titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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Datasheets/Protocols

80527 Lot 220302-A

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