PARP/PARPTrap™ Screening & Profiling

PARP (Poly ADP-Ribose Polymerase) proteins are a large family of 17 members that catalyze the ADP-ribosylation of proteins and DNA. PARPs are part of a network of 150 proteins involved in the DNA damage response, which constantly scan and repair DNA to maintain genome integrity. The PARP proteins are involved in a wide range of biological functions: DNA repair, chromatin remodeling, mitotic spindle assembly, regulation of RNA turnover, regulation of gene expression, apoptosis, and more.

Accelerate your drug discovery and development projects using PARP or PARPtrap™ screening services. We offer the largest PARP proteins panel on the market, allowing you to compare efficacy across our entire PARP family.

PARP and PARPTrap™ Screening and Profiling Services

Our team of experts, using our in-house developed biochemical assay kits, will:

  • Screen for PARP inhibitors from large compound libraries, measured by ELISA, AlphaLISA®, or Fluorescence Polarization assays
  • Screen for PARG inhibitors
  • Compare potency with IC50 screens
  • Answer your questions and guide your project in a timely manner
  • Deliver detailed results promptly and on time

If follow-up studies are required, we can provide the same active proteins as the ones used in our assays.

Learn more about the process and deliverables.

Enzymatic Activity by ELISA

Enzymatic Activity by ELISA

ELISA: Histone proteins are coated on a plate. Biotin-labeled NAD+ is added with the PARP enzyme. PARP-mediated ribosylation activity is detected by adding Streptavidin-HRP (horseradish peroxidase), which binds to the newly formed ribosylation branch, and a chemiluminescent or colorimetric HRP substrate.

Enzymatic Activity by AlphaLISA®

Enzymatic Activity by AlphaLISA®

AlphaLISA® Assay: a PARP enzyme is incubated with biotinylated histone substrate and NAD+ for an hour. An ADP-ribose binding reagent is added with an acceptor bead, then a streptavidin-conjugated donor bead is added. Excitation of the donor bead results in excitation of the acceptor bead and light emission. This is a homogeneous assay.

PARP Trapping

PARP Trapping

Fluorescence Polarization Assay: The assay uses fluorescent DNA probes that emit differently polarized light depending on PARP binding. Addition of a PARP inhibitor prevents ribosylation and results in the trapping of PARP onto the fluorescent oligonucleotide duplex, which increases the Fluorescence Polarization signal in a dose dependent manner. This is a homogeneous assay.

Molecular Degrader Optimization

Molecular Degrader Optimization

AlphaLISA® Assay: A degrader of interest interacts with both PARP and CRBN, bringing them in proximity. PARP-GST is recognized by the GSH donor bead, while CRBN-FLAG binds to anti-FLAG conjugated to the acceptor bead. Excitation of the donor bead results in excitation of the acceptor bead and light emission. This is a homogeneous assay.

Available Service Assays

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*Alternative assay formats may be available. Inquire with our team to learn more. Export results View all selections

Note: Not all targets will have a commercially available reference control. Please inquire with our team if you need to furnish your own controls for specific targets.