PAK5 Recombinant

Catalog #

40077

$515 *

Size: 10 µg

Qty

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Description

Recombinant human PAK5 (p21 (RAC1) activated kinase 5), encompassing amino acids 425-719 containing the Serine/threonine-protein kinase domain. The protein was purified to homogeneity and is kinase active.

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Synonyms

Serine/threonine-protein kinase PAK 5, p21-activated kinase 5, PAK-5, p21-activated kinase 7, PAK-7, KIAA1264, PAK7

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SDS-Page Analysis

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.

34 kDa

Amino Acids

425–719

Specific Activity

4,199 pmole/min/μg

Genbank #

NM_177990

UniProt #

Q9P286

References

1. Li,Y., et al., PLoS ONE 3 (7), E2707 (2008).
2. Ewing,R.M., et al., Mol. Syst. Biol. 3, 89 (2007).

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen): Different concentrations of PAK5 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature. Fluorescence was detected using λ exc=460±40 nm and λem=528±20 nm filters.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40077 Lot 220301

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