PDE10A1 Assay Kit

Catalog #: 60385
Size: 384 reactions
$690
Background:
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE10A is a dual substrate PDE highly expressed in striatal medium spiny neurons. PDE10A inhibitors can improve the cognitive symptoms of schizophrenia, and exhibit potential therapeutic value for Huntington’s disease. PDE10A1 is located in the cytosol, whereas PDE10A2 is a membrane-associated protein.
Description:
The PDE10A1 Assay Kit is designed for identification of PDE10A1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A1 to the binding agent. The key to the PDE10A1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE10A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.
Supplied As:
The PDE10A1 inhibitor screening assay kit comes in a convenient 384-well format, including purified PDE10A1 enzyme, fluorescently labeled PDE10A1 substrate (cAMP), binding agent, and PDE assay buffer for 400 enzyme reactions.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
1) Nishi, A., and Snyder, G.L. J. Pharmacol. Sci., 2010, 114: 6-16.
2) Omori, K., and Kotera, J. Circulation Res. 2007; 100: 309-327.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Phosphodiesterase
Product Type:
Assay Kit
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Product Datasheets

60385.pdf