MUSK, His-tag Recombinant

Catalog #

40079

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human MUSK, GenBank Accession No. NM_005592, a.a. 530-869 (end), with N-terminal His-tag, MW = 40 kDa, expressed in Baculovirus infected Sf9 cell expression system.

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Synonyms

MUSK, muscle, skeletal, receptor tyrosine kinase, Muscle-Specific Kinase Receptor

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Product Data Gallery

Specific Activity

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥66%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 225 mM Imidazole.

40 kDa

Amino Acids

530-869 (end)

Genbank #

NM_005592

UniProt #

O15146 Q13255

Tag(s)

N-terminal His-tag

References

1. Zhou H. et al., J. Cell Biol. 146 (5), 1133-1146 (1999).
2. Evoli A. et al., Ann. N. Y. Acad. Sci. 1132, 76-83 (2008).

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done using ADP-Glo (Promega) reagents with 100 µM ATP, 20 µM acetyl-CoA and 30 mM NaHCO3 in 25 µL kinase assay buffer (30 mM HEPES pH=7.4, 2 mM MgCl2, 1 mM DTT, and 2 mM Sodium citrate). Substrate used was MBP. Reaction was performed at 30˚C for 40 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40079.pdf

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