MSSK1 (SRPK3), GST-tag Recombinant

Catalog #

40132

$410 *

Size: 10 µg

Qty

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Description

Recombinant human MSSK1 (Muscle-specific serine kinase 1, also known as SRPK3), full length. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is kinase-active.

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Synonyms

Muscle-specific serine kinase 1, STK23, MSSK-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

MSSK1 (GST-Full Length)

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

98 kDa

Amino Acids

full length

Specific Activity

210 pmol/min/µg

Genbank #

BC117124

UniProt #

Q9UPE1

Tag(s)

N-terminal GST-tag

Background

MSSK1, also known as SRPK3, is a muscle-specific protein kinase belonging to the serine arginine protein kinase family which phosphorylates serine/arginine repeat-containing proteins. Heart and skeletal muscle show high expression of the MSSK1/SRPK3 gene product. SRPK3-null mice display a new entity of type 2 fiber-specific myopathy with a marked increase in centrally placed nuclei. Transgenic mice overexpressing SRPK3 in skeletal muscle show severe myofiber degeneration and early lethality (1).

References

Nakagawa, O. et al: Centronuclear myopathy in mice lacking a novel muscle-specific protein kinase transcriptionally regulated by MEF2. Genes Dev. 2005 Sep 1;19(17):2066-77.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Kinase activity was measured using PKCtide sequence ERMRPRKRQGSVRRRV (stock solution 1 mg/ml dissolved in distilled water).
Increasing amounts of kinase were mixed with a final concentration of 200 µg/ml peptide substrate in a buffer consisting of 5 mM MOPS pH 7.2, 5 mM MgCl2, 2.5 mM β-glycerol-phosphate, 1 mM EGTA, 0.4 mM EDTA, 50 µg/ml BSA (bovine serum albumin) and 50 µM fresh DTT (final concentrations). The reaction was initiated by adding [33P]-ATP (1 µCi/sample) mixed with non-radioactive ATP (final concentration of 10 µM). The blank was determined from a “no substrate” sample.
The reaction was incubated for 15min at 30°C and terminated by spotting 20 μl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40132 Lot 220127

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MSSK1 (SRPK3), GST-tag Recombinant

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