MGLL, His-tag, GST-tag Recombinant

Catalog #

71096

$370 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human monoglyceride lipase (MGLL), GenBank Accession No. NM_007283, a.a. 2-313(end), with N-terminal His-tag and N-terminal GST-tag, MW=62 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

monoglyceride lipase, MGLL, HUK5, MAGL, Monoacylglycerol Lipase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 228 mM imidazole, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

62 kDa

Amino Acids

2–313(end)

Genbank #

NM_007283

UniProt #

Q99685

Tag(s)

N-terminal His-tag, N-terminal GST-tag

Background

Monoacylglycerol lipase (MAGL) catalyzes hydrolysis of intracellular triglyceride stores, forming fatty acids and glycerol. It is a key enzyme in the metabolism of 2-arachidonoylglycerol, hence is involved in a similar pathway to DAGL. MAGL is localized in the enteric nervous system where it functions to regulate levels of endocannabinoids, and as a result, regulates intestinal motility. It is also found in the gut epithelium where it may have a role in digestion.

References

1. Sun, H., et al., Oncogene 2013;32(2): 234-241.
2. Labar, G., et al., Chembiochem. 2010;11(2):218-227.

Storage/Stability

>6 months at –80°C

Assay Conditions

Reaction was performed in 50 µl reaction mix containing 50 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM 4-Methylumbelliferyl butyrate, and various amounts of MGLL. Reaction was incubated at 37°C for 15 minutes and fluorescent signal was measured at ex 360 nm, em 445 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Metabolic Enzymes-Transferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71096_Lot130116.pdf

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MGLL, His-tag, GST-tag Recombinant

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