PRMT4 Chemiluminescent Assay Kit

Catalog #: 52041L
Size: 96 reactions
PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.
The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1
Supplied As:
Kit comes in a convenient format, with 8-well strips precoated with the specific PRMT4 peptide substrate, reagents, and purified PRMT4 enzyme for 96 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt


Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Dillon S.C., et al. 2005. Genome Biology 6:227.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets