MELK, GST-tag Recombinant

Catalog #

40708

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human MELK, Genbank Accession #NM_014791, a.a. 1- 340, with an N-terminal GST-tag, MW = 61 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

Maternal embryonic leucine zipper kinase, Protein kinase Eg3, PK38, Protein kinase PK38

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 Cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

61 kDa

Amino Acids

1-340

Specific Activity

200 pmol/min/µg.

Genbank #

NM_014791

UniProt #

Q14680

Tag(s)

N-terminal GST-tag

References

1. Nakano, I., et al., J Cell Biol 2005;170:413-27.
2. Lin, ML., et al., Breast Cancer Res. 2007;9:R17.

Storage/Stability

>6 months at -80°C.

Assay Conditions

5 mM MOPS, 2.5 mM Β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 5 μg of ZIPtide synthetic peptide substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter. Application: Useful for

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Serine-Threonine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40708_Lot140722.pdf

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