MAPK10 (JNK3), GST-tag Recombinant

Catalog #

40092

$355 *

Size: 10 µg

Qty

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Description

Human MAPK10 (mitogen-activated protein kinase 10), also known as PRKM10, JNK3 (c-Jun N-terminal kinase-3), or SAPKβ (stress activated protein kinase-β), GenBank Accession No. NM_002753, a.a. 2-422 (end), with N-terminal GST-tag, MW=75 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

MAPK10, mitogen-activated protein kinase 10, JNK3, c-Jun N-terminal kinase 3, SAPKβ, stress activated protein kinase beta, PRKM10

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Product Data Gallery

MAPK10 Activity

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.

75 kDa

Amino Acids

2–422 (end)

Specific Activity

22 pmol/min/µg

Genbank #

NM_002753

UniProt #

P53779

Tag(s)

N-terminal GST-tag

References

1. Lisnock J, et al. (2000). Biochemistry 39(11): 3141–8.
2. Hu MC, et al. (1997). Oncogene 15(19): 2277–87.
3. Ying J, et al. (2006). Leukemia 20(6): 1173-1175.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction was performed in 50 µl kinase reaction buffer (40 mM Tris pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.5 mM DTT) containing 50 µM ATP, 0.2 mg/ml ATF2 (BPS #40520) and 2-16 ng/µl MAPK10 for 30 minutes at 30°C. The amount of ATP transferred was assayed using the BPS Bioscience ATP quantification reagents.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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MAPK10 (JNK3), GST-tag Recombinant

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