LDLR, FLAG-Avi-Tag, Biotin-Labeled Recombinant

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Catalog #

71206

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Description

Recombinant human LDLR (Low Density Lipoprotein Receptor), encompassing amino acids 22-788. This construct contains a C-terminal FLAG-tag followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Synonyms

FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PCSK9-LDLR[Biotinylated] Interaction

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Human

Construct

LDLR (22-788(end)-FLAG-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

88 kDa + glycans

Amino Acids

22–788(end)

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_000527

UniProt #

P01130

Tag(s)

C-terminal FLAG and Avi tags

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be >90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of LDLR, inducing LDLR degradation.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to PCSK9 [Biotinylated]-LDLR Binding Assay Kit (BPS Bioscience #72002) with PCSK9 coated at 4 ng/µl and LDLR[Biotinylated] titrated at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cholesterol-Related Proteins

Regulatory Status

Research Use Only

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Datasheets/Protocols

71206 Lot 230301

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LDLR, FLAG-Avi-Tag, Biotin-Labeled Recombinant

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