LDLR, FLAG-tag Recombinant

Catalog #

71205

$465 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human low density lipoprotein receptor (LDLR protein), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_000527, a.a. 22-788(end), with C-terminal FLAG-tag, MW=87.4 kDa* (calculated), expressed in an HEK293 cell expression system.

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Synonyms

FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293 cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol, 100µg/mL FLAG peptide.

87.4 kDa

Amino Acids

22–788(end)

Genbank #

NM_000527

UniProt #

P01130

Tag(s)

C-terminal FLAG-tag

Background

The low density lipoprotein receptor (LDLR protein) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the LDLR protein, inducing LDLR degradation.

References

1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1,8:9.
2. Qian, YW., et al., J Lipid Res. 2007 Jul,48(7):1488-98.
3. Fasano, T., et al., Athersclerosis. 2009 Mar,203(1):166-71.

Storage/Stability

>6 months at -80°C.

Assay Conditions

100 ng/well of LDLR in 50 µl (overnight at 4°C). Initiated binding reaction with addition of various concentrations of PCSK9-biotin (50 ng/well is the established kit condition). Incubated for 2 hours at room tempterature. Detected binding with Strep-HRP generated luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and screening small molecules for drug discovery.

Shipping Temperature

-80°C

Notes

* LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cholesterol Related

Regulatory Status

Research Use Only

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Datasheets/Protocols

71205_Lot_170223-2_.pdf

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LDLR, FLAG-tag Recombinant

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