LDHB, His-tag Recombinant

Catalog #

71078

$370 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human lactate dehydrogenase B (LDHB) a.a. 2 – 334 (end) (GenBank Accession No. NM_002300) with N-terminal His-tag, MW = 37.5 kDa, expressed in an E. coli expression system.

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Synonyms

lactate dehydrogenase B, LDHB, LDH

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 240 mM imidazole, 0.04% Tween-20, and 20% glycerol

37.5 kDa

Amino Acids

2 – 334 (end)

Specific Activity

≥350 nmole/min/µg.

Genbank #

NM_002300

UniProt #

P07195

Tag(s)

N-terminal His-tag

References

1. Armstrong, A.J., et al. J Clin Oncol. 2012 Sep 30;30(27):3402-7.
2. Sudo, K., et al., Hum Genet. 1992 May;89(2):158-62.

Storage/Stability

>6 months at –80°C

Assay Conditions

Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHB in a volume of 200 µL. Initiate reactions with LDHB. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. using Tecan Infinite 1000.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Dehydrogenase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71078.pdf

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