LAR (PTPRF), GST-tag

Catalog #: 30046
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 58 kDa
Genbank #: NM_130440
Tag(s): N-terminal GST-tag
a.a: 1330–1620
Background:
PTPRF or LAR is a member of the protein tyrosine phosphatase family with an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. PTPRF has been shown to function in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance.
Description:
Human transmembrane leukocyte antigen related PTP (LAR), GenBank accession No. NM_130440, a.a. 1330-1620 (catalytic domain) with N-terminal GST-tag, MW=58 kDa, expressed in an E. coli expression system.
Synonym(s):
LAR, PTPRF, Leukocyte Antigen-Related PTP
Specific Activity:
0.7 U/µg
Unit Definition:
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions:
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 160 ng/µl LAR. Assay was done at 30&Deg;C for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. H. Cho et al. Biochemistry 1991, 30: 6210-6216.
2. H. Cho et al. Biochemistry 1992, 31: 133-138.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Receptor
Product Type:
Recombinant Protein
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