Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; JAK1, JAK2, JAK3 and Tyk2. Various studies have suggested that regulation of JAK activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The JAK1 (Janus Kinase 1) Assay Kit is designed to measure JAK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Need us to run inhibitor screens or profile your compounds against JAK1? Check out our Kinase Screening Services.
The JAK1 (Janus Kinase 1) Assay Kit comes in a convenient 96-well format, with enough purified recombinant JAK1 enzyme, JAK1 substrate peptide (IRS-1tide), ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
Kinase-Glo MAX (Promega #V6071) Dithiothreitol (DTT, 1 M) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator
Alicea, N.L. et al., Current Drug Targets12(4):546-555 (2011)
Storage/Stability
Up to 6 months when stored as recommended.
Instructions for Use
See assay kit data sheet for detailed protocol
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.