c-Met Kinase Assay Kit

Catalog #: 79559
Size: 96 reactions
$495
Background:

c-Met, also known as HGFR (hepatocyte growth factor receptor), is a receptor protein tyrosine kinase encoded by the gene MET. Upon binding its ligand HGF (hepatocyte growth factor), c-Met activates multiple cellular processes including proliferation, adhesion and angiogenesis. Importantly, many studies report that c-Met is overexpressed in various carcinomas, suggesting that targeting HGF/c-Met signaling could be a promising target for cancer treatment.

Description:

The c-Met Kinase Assay Kit is designed to measure c-Met kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Materials Required But Not Provided:

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Synonym(s):
HGFR, hepatocyte growth factor receptor
Supplied As:
The c-Met Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Met enzyme, c-Met substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

Storage / Stability:
Up to 6 months when stored as recommended.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Sierra J. R., Ther. Adv. Med. Oncol. 3(1 Suppl): S21–S35 (2011)

Warning(s):
Avoid freeze/ thaw cycles
Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79559.pdf