B-Raf(V600E) Kinase Assay Kit

Catalog #: 48688
Size: 96 reactions
$495
Description:
B-raf, after stimulation by activated RAS, phosphorylates MEK1 resulting in activation of the mitogen-activated protein (MAP) kinase cascade. Since the MAPK cascade has been shown to be a critical factor in melanoma development, it has been proposed that targeting B-raf could be a promising approach to combat melanoma. More importantly, 40-60% of melanomas display a mutation in BRAF, e.g. B-Raf(V600E), which leads to constitutive activation of the MAP kinase pathway. The B-Raf(V600E) Kinase Assay Kit is designed to measure B-Raf(V600E) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. In addition, the B-Raf(V600E) Kinase Assay Kit includes wild type B-Raf enzyme as a positive control for researchers investigating B-Raf(V600E) specific inhibitors.
Synonym(s):
BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF (V600E)
Supplied As:
The B-Raf(V600E) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant B-Raf enzyme, B-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Cantwell-Dorris, ER, et al. Mol Cancer Ther. 2011 Mar;10(3):385-94

Obaid NM, et al. Int J Mol Sci. 2017 Mar 8;18(3). pii: E585.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

48688.pdf