JARID1B (KDM5B) Homogeneous Assay Kit

Catalog #: 50512
Size: 384 reactions
$1,950
Background:
JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
Description:
The JARID1B Homogeneous Assay Kit is designed to measure activity of the JARID1B for screening and profiling applications. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
JARID1B, PLU-1, KDM5B, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Supplied As:
The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 384 enzyme reactions.
Contraindications:
Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
>6 months at –80°C
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Lahoud, M.H., et al. Genome Res 11 (8): 1327–34.
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50512.pdf