JARID1B (KDM5B, PLU-1), His-FLAG-tags Recombinant

Catalog #

50121

$465 *

Size: 20 µg

Qty

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Description

Human JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_006618), a.a. 2-751 with N-terminal His-FLAG-tag, MW=88 kDa, expressed in Sf9 cells via a baculovirus expression system.

This product has been cited 1 time.

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Synonyms

JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, CT31, Cancer/Testis Antigen 31, PLU-1, Jumonji, AT Rich Interactive Domain 1B, Lysine-Specific Demethylase 5B

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JARID1B (KDM5B) inhibition curve using an AlphaLISA assay (#50512-2). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 µg/mL FLAG peptide and 20% glycerol.

88 kDa

Amino Acids

2–751

Specific Activity

0.1 pmole/min/μg

Genbank #

NM_006618

UniProt #

Q9UGL1

Tag(s)

N-terminal His-FLAG-tags

Background

Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

References

1. Yamane K, et al. (2007). Mol. Cell 25(6): 801–12.
2. Barrett A, et al. (2007). Int. J. Cancer 121(2): 265–75.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM a-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1B (20 – 100 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Demethylase

Regulatory Status

Research Use Only