Human Interleukin-4 Recombinant
Catalog #
90193-A
$165
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Description
Recombinant human Interleukin-4 is a disulfide-linked monomer protein consisting of 130 amino acid residues, migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-4 mature chain was expressed in E. coli.
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Synonyms
IL-4, interleukin 4, BCGF1, B cell Growth Factor-1, IL4
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥95% by SDS-PAGE and HPLC
Format
lyophilized protein
Formulation
Lyophilized from a 0.2 µm filtered solution of 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.
MW
15 kDa
Endotoxin Level
Endotoxin level was found to be < 0.1 ng/μg (1 EU/μg), using the LAL gel clot method.
Biological Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of > 1x 107 units/mg.
Genbank #
P05112
UniProt #
P05112
Background
Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) that are the biologically most active helper cells for B-cells and that also secrete IL-5 and IL-6. The biological activities of IL-4 are mediated by a specific receptor. The extracellular domain of the IL-4 receptor is related to the receptors for EPO, IL-6, and the beta chain of the IL2 receptor (CD124). Two types of IL-4 receptor (IL-4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL-4R-alpha. The type 2 receptor is a heterodimer consisting of IL-4Ralpha and IL-13Ralpha1. IL-4 enhances expression of MHC class 2 antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. Pretreatment of macrophages with IL-4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. IL-4 synergises with EPO and G-CSF in the generation of colonies containing granulocytes or erythroid progenitor cells in a colony formation assay.
References
1. Tachdjian R, et al. J. Exp. Med., Sep 2009, 206: 2191 - 2204.
2. Yu, M., et al. J Biol Chem. 2009 Nov 20,284(47):32968-79. .
3. Ennaciri J, Girard D. J Immunol. 2009 Oct 15,183(8):5261-9
2. Yu, M., et al. J Biol Chem. 2009 Nov 20,284(47):32968-79. .
3. Ennaciri J, Girard D. J Immunol. 2009 Oct 15,183(8):5261-9
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