IL-4/IL-13 Responsive STAT6 Luciferase Reporter HEK293 Cell Line

Catalog #

78941

$7,250 *

Size: 2 vials

Qty

Bulk/Custom

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Description

IL-4/IL-13 Responsive STAT6 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line with stably integrated Firefly luciferase reporter under the control of STAT6 response element and human STAT6 (signal transduced and activator of transcription factor 6), resulting in a fully functional STAT6 signaling pathway.

This cell line has been validated with activation assays with human IL-4 (interleukin-4) and IL-13 (interleukin-13) and the use of anti-IL-4R and anti-IL-13R antagonists.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of IL-4 or IL-13 signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

STAT-6

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Product Data Gallery

Reporter activation of IL-4/IL-13 Responsive STAT6 Luciferase Reporter HEK293 Cell Line in response to human IL-4 and IL-13

Inhibition of hIL-4-induced reporter activity by Dupilumab Recombinant Human Monoclonal Antibody in IL-4/IL-13 Responsive STAT6 Luciferase Reporter HEK293 Cell Line.

Inhibition of hIL-13-induced reporter activity by Lebrikizumab in IL-4/IL-13 Responsive STAT6 Luciferase Reporter HEK293 Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1M	BPS Bioscience #79723

Materials Used in Cellular Assay

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Human Interleukin-4	BPS Bioscience #90193
Human Interleukin-13	BPS Bioscience #90179
Dupilumab Recombinant Human Monoclonal Antibody	Invitrogen #MA5-41985
Lebrikizumab	MedChem #HY-P99025
Clear-bottom, white 96-well tissue culture-treated plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The transcription factor STAT6 (signal transduced and activator of transcription factor 6) plays a vitally important role in immune cells. IL-4-activated STAT6 signaling is required for Th2 differentiation and immunoglobulin class switching and it also mediates direct repression of inflammatory enhancers in macrophages. STAT6 is involved in several aspects of allergic inflammatory disease, like airway hyperresponsiveness, eosinophilic infiltration, and mast cells responses. STAT6 is activated primarily by IL-4 and the related IL-13, with have overlapping biologic profiles. Upon IL-4 and IL-13 binding, the receptor complex composed of IL-4Ralpha and IL-13Ralpha1 activates the receptor-associated Janus kinases (JAK1 and Tyk2), leading to the activation of STAT6. Activated STAT6 forms homodimers that translocate to the nucleus where they bind the promoter of responsive genes, inducing gene transcription. While both cytokines activate STAT6 signal, it has been proven that IL-4 is more potent in engaging this signaling pathway than IL-13. Dysfunction of the STAT6 pathway leads to allergic reactions (asthma, atopic dermatitis, food allergies) and cancer (Hodgkin, central nervous system, and follicular lymphoma). STAT6 can serve as a diagnostic and prognostic tool. Inhibitors have been shown to reduce acute inflammation in animal models, and future developments may lead to new therapeutical tools for STAT6 related pathologies.

References

Kasaian MT., et al., 2013. Am J Respir Cell Mol Biol. 49(1):37-46.
Czimmerer Z., et al., 2018. Immunity 48(1):75-90.e6.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture and Functional Cellular Assay
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1M (BPS Bioscience #79723): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 μg/ml of Geneticin and 0.5 μg/ml Puromycin

Applications

Screen and characterize activators or inhibitors of the STAT6 signaling pathway.
Screening anti-IL-4, anti-IL-4R, anti-IL-13, or anti-IL-13R antibodies.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Signaling

Regulatory Status

Research Use Only