IKKβ, GST-Tag Recombinant

Catalog #

40304

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human IKKβ (inhibitor of nuclear factor kappa B kinase subunit beta), full length encompassing amino acids 2-756 (end). This construct contains an N-terminal GST-tag. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

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Synonyms

NF-kappa-B inhibitor kinase beta, IKK2, IKBKB, IKKB, NFKBIKB

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

IKKβ Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

IKKβ (GST-2-756(end))

Host Species/Expression System

Sf9

Purity

≥75%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

113 kDa

Amino Acids

2-756

Specific Activity

≥40 pmol/min/μg

Genbank #

NM_001556

UniProt #

O14920

Tag(s)

N-terminal GST-tag

References

1. Woronicz, J.D. et al., Science. 1997; 278: 866-869.
2. Yin, M.J., et al., Nature. 1998; 396: 77-80.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done using the IKKβ Kinase Assay Kit (BPS Bioscience #78507) with various amounts of IKKβ .

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling,

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Serine Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40304 Lot220216-G2

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