SHP-2 (catalytic domain) Homogeneous Assay Kit

Catalog #

79317

$440 *

Size: 96 reactions

Qty

Bulk/Custom

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Description

Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. SHP-2 (PTPN11) is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

The SHP-2 (catalytic domain) Homogeneous Assay Kit is a complete assay system designed to measure SHP-2 activity for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is needed to analyze the SHP-2 activity level. The fluorogenic substrate, DiFMUP, is incubated with purified SHP-2 and the enzymatic activity releases DiFMU fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against SHP-2 (catalytic domain)? Check out our Protein Tyrosine Phosphatase Screening Services.

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Synonyms

SHP2, CFC, NS1, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, MGC14433, SH2, SH 2, SH-2, SHP, shp

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

Ki comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SHP-2 activity measurements. In addition, the kit includes purified SHP-2 enzyme and SHP-2 inhibitor, orthovanadate, for use as a positive and negative control

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading exc/em=360nm/460nm

Format

Catalog Number	Component	Amount	Storage
30022	Recombinant Human SHP-2	1 µg	-80°C	*Avoid freeze/ thaw cycles!*
79769	1mM SHP-2 Substrate (DiFMUP)	50 µl	-80°C
79626	5X SHP-2 Assay Buffer	3 ml	-20°C
	1mM Na₃VO₄	20 μl	-80°C
79685	Black, low binding black microtiter plate	1	Room Temperature

UniProt #

Q06124

References

Chai, J., et al., Cell, 2001 Mar 9;104(5):769-80.
Denault, JB., and Salvesen, GS., J. Biol. Chem., 2003 Sep 5;278(36):34042-50.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protein phosphatase

Regulatory Status

Research Use Only

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SHP-2 (catalytic domain) Homogeneous Assay Kit

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