GLS1, His-tag Recombinant
Catalog #
71102
$555
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Description
Human glutaminase transcript variant 1 (GLS1), also known as GAC, GAM and KGA, GenBank Accession No. NM_014905, a.a. 17-669(end), with N-terminal His-tag, expressed in an E. coli cell expression system. MW=73 kDa.
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Synonyms
glutaminase, glutaminase transcript variant 1, GLS1, GAC, GAM, KGA, GLS
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Product Data Gallery
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
80%
Supplied As
Aqueous buffer solution.
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.
MW
73 kDa
Amino Acids
17–669(end)
Specific Activity
≥80%
Genbank #
NM_014905
UniProt #
O94925
Background
GLS1 is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. GLS1 is primarily expressed in the brain and kidney where it catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
References
1. Van den Heuvel, A.P., et al., Cancer Biol. Ther. 2012;13(12):1185-1194.
2. Thangavelu, K., et al., Proc. Natl. Acad. Sci. USA 2012;109(2):7705-7710.