The GAL4 Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter and can be used alongside BPS Cat. #60655 as a control.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of GAL4-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Mycoplasma Testing
The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit
(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage/Stability
Immediately upon receipt, store vials in liquid nitrogen.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Cat. #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Cat. #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Shipping Temperature
-80°C (dry ice)
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.