EZH2/EED, His-tag, FLAG-tag Recombinant

Catalog #

51002

$480 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 87 kDa, and human EED (GenBank Accession No. NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, co-expressed in a baculovirus expression system.

This product has been cited 1 time.

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Synonyms

KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2; Embryonic Ectoderm Development, hEED, EED, WAIT-1, EZH

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 200 mM imidazole, and 20% glycerol.

138 kDa (complex)

Amino Acids

2–end (EZH2 and EED)

Genbank #

NM_004456 (EZH2), NM_003797(EED)

UniProt #

Q15910

Tag(s)

N-terminal His-tag (EZH2), N-terminal FLAG-tag (EED)

Background

EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

References

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).