EZH2 (Y641F) Chemiluminescent Assay Kit

Catalog #
52075
$1,550 *
Size: 96 reactions
Qty
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Description

The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (Y641F) Chemiluminescent Assay Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 (Y641F) complex for 96 enzyme reactions. The key to the EZH2 (Y641F) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonyms
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Materials Required But Not Supplied

TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format
Catalog # Component Amount Storage
51017 EZH2 (Y641F)/EED/SUZ12/ RbAp48/AEBP2 50 µg -80°C Avoid freeze/
thaw cycles!
52120 20 μM S-adenosylmethionine 250 µl -80°C
52140X Primary Antibody 24 12.5 µl -80°C
52131H Secondary HRP-labeled Antibody 2 10 µl -80°C
52170B 4x HMT Assay Buffer 2B 3 ml -20°C
52100 Blocking Buffer 4 50 ml +4°C
  HRP chemiluminescent substrate A (translucent bottle)
6 ml

+4°C
  HRP chemiluminescent substrate B (brown bottle)
6 ml

+4°C
  96-well plate precoated with histone substrate
1 plate

+4°C
UniProt #
Q15910
References
1. Dillon, S.C., et al. Genome Biology 2005; 6:227. 2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.