EZH2 (Y641N) Chemiluminescent Assay Kit

Catalog #

52076

$1,550 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against EZH2 (Y641N)? Check out our Methyltransferase Screening Services.

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Synonyms

KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with 8-well strips precoated with histone H3

Format

COMPONENTS

UniProt #

Q15910

References

1. Dillon, S.C., et al. Genome Biology 2005; 6:227.

2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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EZH2 (Y641N) Chemiluminescent Assay Kit

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