EZH2 (Y641N) Chemiluminescent Assay Kit

Catalog #: 52076
Size: 96 reactions
$1,450
Description:
The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
UniProt
Q15910
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Supplied As:
Kit comes in a convenient format, with 8-well strips precoated with histone H3
Format:

COMPONENTS

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):

1. Dillon, S.C., et al. Genome Biology 2005; 6:227.

2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
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Product Datasheets

52076.pdf