EZH2 (Y641C) /EED/SUZ12/RbAp48/AEBP2, His-tag

Catalog #: 51029
Size: 20 µg
$375
Species: Human
Host Species: Sf9 insect cells
MW: 325 kDa (complex)
Genbank #: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Tag(s): N-terminal His-tag
a.a: 2–end (all components)
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Mutant version of our regular EZH2 5-member complex (Cat. #51004), but with a Tyr-to-Cys mutation on a.a. 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end; Y646C*, with N-terminal His tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.
UniProt
Q15910
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH2 (Y646C), EZH, Retinoblastoma Binding Protein 4 (RBBP4)
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

≥ 6 months at -80°C

Application(s):
Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
Reference(s):

1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Notes:
This mutation is often cited in the scientific literature as Y641C in variant 3 (GenBank Accession No. NM_001203247).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51029_Lot100907.pdf