DYRK1B Kinase Assay Kit

Catalog #

78401

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The DYRK1B Kinase Assay Kit is designed to measure DYRK1B kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

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Synonyms

DYRK1B, Dual specificity tyrosine-phosphorylation-regulated kinase 1B, Minibrain-related kinase, Mirk protein kinase, MIRK

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The assay kit comes in a convenient 96-well format, with enough purified recombinant DYRK1B kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
DTT (Dithiothreitol), 1M (optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
100646	DYRK1B, GST-tag*	3 µg	-80°C
79334	Kinase assay buffer 1 (5x)	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
78393	DYRK-tide (10 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

UniProt #

Q9Y463

Background

DYRK1B is a dual-specificity kinase with serine/threonine and tyrosine kinase activities. It is activated as part of the cellular response to DNA damage and regulates gene transcription, notably by increasing the activity of transcription factors such as TCF1 or FOXO1. DYRK1B may play a role in several pathologies as a result of its metabolic activities. It also represents a potential therapeutic target in several types of cancer.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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DYRK1B Kinase Assay Kit

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