DYRK2 Kinase Assay Kit

Catalog #

78392

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The DYRK2 Kinase Assay Kit is designed to measure DYRK2 kinase activity for screening and profiling applications using Kinase-Glo^® MAX as a detection reagent.

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Synonyms

DYRK2, Dual specificity tyrosine-phosphorylation-regulated kinase 2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The assay kit comes in a convenient 96-well format, with enough purified recombinant DYRK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40053	DYRK2*	3 µg	-80°C
79334	Kinase assay buffer 1 (5x)	1.5 ml	-20°C
79686	ATP (500 µM)	1.5 ml	-20°C
78393	DYRK-tide (10 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #

Q92630

Background

DYRK2 (dual specificity tyrosine phosphorylation regulated kinase 2) is a serine/threonine kinase as well as a tyrosine kinase implicated in the control of cell proliferation, cell cycle progression, and cytoskeleton organization. DYRK2 has demonstrated tyrosine autophosphorylation activity. It is notably activated by ATM in response to DNA damage and phosphorylates serine 46 on tumor suppressor TP53, leading to apoptosis. Many other targets have been identified, including various ligases involved in ubiquitination and ubiquitin-dependent proteasomal degradation of proteins. The kinase indisputably plays a role in various types of cancer, although its exact function and mechanism of action in cancer cells remains somewhat elusive [1].

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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DYRK2 Kinase Assay Kit

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