Cereblon/DDB1/Cul4A/Rbx1 Complex Recombinant

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Catalog #

100329

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Description

Complex of CRBN (Cereblon), DDB1(damage specific DNA binding protein 1), Cul4A (cullin 4A), and Rbx1 (ring-box 1) proteins. These recombinant proteins are co-expressed and affinity purified. Recombinant human CRBN, encompassing amino acids 2-442(end). This construct contains an N-terminal Flag-tag. Recombinant human DDB1, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. Recombinant human Cul4A, encompassing amino acids 2-759(end). This construct contains an N-terminal His-tag (6xHis). Recombinant human Rbx1, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis).

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Synonyms

CRBN, Protein X 0001, MRT2A MRT2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Cereblon-BRD3 Activity

Cereblon inhibition curve using a TR-FRET assay (#78301). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

Cereblon (FLAG-2-442(end)) /DDB1 (FLAG-2-1140(end)) / Rbx1(M5I) (His-2-108(end)) / Cul4A (His-2-759(end))

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol

Cereblon: 51 kDa; DDB1: 128 kDa; Cul4a: 88 kDa; Rbx1: 13 kDa

Amino Acids

Cereblon: 2-442(end); DDB1: 2-1140(end); Cul4a: 2-759(end); Rbx1: 2-108(end)

Genbank #

Cereblon: NM_016302; DDB1: NM_001923; Cul4a: NM_003589; Rbx1: NM_014248

UniProt #

Cereblon: Q96SW2; DDB1: Q16531; Cul4a: Q13619; Rbx1: P62877

Tag(s)

Cereblon: N-terminal FLAG-tag; DDB1: N-terminal FLAG-tag; Cul4a: N-terminal His-tag; Rbx1: N-terminal His-tag

References

1. Gang, Lu., et al., Science. 2014; 343(6168): 305-309.

2. Zhu, Y.X., et al., Blood. 2011; 118: 4771-4779.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to PROTAC Optimization Kit for BET Bromodomain-Cereblon Binding (BPS Bioscience #79770) with Cereblon complex titrated from 250 ng/rxn to 0 ng/rxn

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

E3 Ligase

Regulatory Status

Research Use Only

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Datasheets/Protocols

100329 Lot 220131-1

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Cereblon/DDB1/Cul4A/Rbx1 Complex Recombinant

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