Rbx1/Cul4B/Dcaf11/Ddb1 Complex Recombinant
Complex of Rbx1 (Ring-Box 1), Cul4B (cullin 4B), Dcaf11 (Ddb1- and Cul4-associated factor 11) and Ddb1 (Damage specific DNA binding protein 1. Recombinant Rbx1, encompassing amino acids 2-108(end), mutated at M5I, contains an N-terminal His-tag (6xHis). Recombinant Cul4B, encompassing amino acids 3-913(end), contains an N-terminal His-tag (6xHis). Recombinant Dcaf11, encompassing amino acids 2-546(end), contains an N-terminal FLAG-tag. Recombinant human Ddb1, encompassing amino acids 2-1140(end) contains an N-terminal FLAG-tag. These recombinant proteins were co-expressed, and affinity purified.
≥85%
Aqueous buffer solution.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol
Eukaryotic cells have evolved complex mechanisms to regulate cellular pathways and protein quality. Cullin proteins are a family of hybrophobic proteins that serve as scaffold and can complex with RING and adaptor proteins to form CRLs (E3 ligase-Cullin-RING ligase) complexes, which can interact with different substrates and regulate multiple cellular processes. When the CRL Rbx1-Cul4b-Ddb1 binds to Dcaf11 it can target p21 for ubiquitination and regulate cell cycle progression.
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