VHL/CUL2/ELOB/ELOC/RBX1 complex of human VHL (GenBank Accession No. NM_000551) a.a. 2-212(end) with N-terminal FLAG-tag and MW= 25 kDa, human CUL2 (GenBank Accession No. NM_003591), a.a. 2-745(end) with N-terminal FLAG-tag and MW= 88 kDa, human ELOB (GenBank Accession No. NM_007108), a.a. 2-118 with N-terminal FLAG-tag and MW= 14 kDa, ELOC (GenBank Accession No. NM_005648), a.a. 2-112(end) with N-terminal His-tag and MW= 13 kDa human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, individually expressed in a HEK293 expression system.
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Synonyms
CUL2, ELOB, ELOC, RBX1, VHL, VBC
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
VHL 5-c BRD3 Interaction
VHL Ubiqitination inhibition curve using a TR-FRET assay (#78305). Representative data only; data may vary by lot.
1. Kamura, T., et al., Science. 1999; 284(5414): 657-661. 2. Stebbins, C.E., et al., Science. 1999; 284(5413): 455-461.
Storage/Stability
At least 6 months at –80°C.
Assay Conditions
ARV-771-mediated binding of VHL
BPS #100373 to BET bromodomain was assessed using
A-screen technology. Various amounts of VHL were
incubated in 10-ul reaction with ARV-771 and GSTBRD3(
BD2) BPS #31033 at room temperature for one
hour. After this, FLAG-acceptor beads and GSH-donor
beads were added followed by detection of A-counts.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
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