Complex of ELOB (Elongin-B), ELOC (Elongin-C) and VHL (von Hippel-Lindau disease tumor suppressor), expressed individually and affinity purified as a complex.
Recombinant human ELOB, full length encompassing amino acids 2-118 (end). This construct contains an N-terminal FLAG-tag. Recombinant human ELOC, full length encompassing amino acids 2-112 (end). This construct contains an N-terminal His-tag (6xHis). Recombinant human VHL, full length encompassing amino acids 2-213 (end). This construct contains an N-terminal FLAG-tag.
1. Kamura, T., et al., Science. 1999; 284(5414): 657-661. 2. Stebbins, C.E., et al., Science. 1999; 284(5413): 455-461.
Storage/Stability
At least 6 months at –80°C.
Assay Conditions
Assay run according to VHL Binding Assay Kit (BPS Bioscience #78805) with ELOB/ELOC/ VHL Complex titrated at various concentrations. This assay uses fluorescence polarization to determine the binding of the complex to a fluorescent probe.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Binding studies and screening of binding inhibitors.
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